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dapi nuclear roi  (Oxford Instruments)


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    Structured Review

    Oxford Instruments dapi nuclear roi
    Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), <t>DAPI</t> (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).
    Dapi Nuclear Roi, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 41318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi nuclear roi/product/Oxford Instruments
    Average 99 stars, based on 41318 article reviews
    dapi nuclear roi - by Bioz Stars, 2026-05
    99/100 stars

    Images

    1) Product Images from "Kat5 cKO Biological Domain Signatures Align with Human Alzheimer’s Disease"

    Article Title: Kat5 cKO Biological Domain Signatures Align with Human Alzheimer’s Disease

    Journal: bioRxiv

    doi: 10.1101/2025.09.13.676046

    Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), DAPI (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).
    Figure Legend Snippet: Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), DAPI (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).

    Techniques Used: Derivative Assay, Staining, Mutagenesis



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    dapi nuclear roi  (Oxford Instruments)
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    Oxford Instruments dapi nuclear roi
    Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), <t>DAPI</t> (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).
    Dapi Nuclear Roi, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi nuclear roi/product/Oxford Instruments
    Average 99 stars, based on 1 article reviews
    dapi nuclear roi - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), DAPI (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).

    Journal: bioRxiv

    Article Title: Kat5 cKO Biological Domain Signatures Align with Human Alzheimer’s Disease

    doi: 10.1101/2025.09.13.676046

    Figure Lengend Snippet: Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), DAPI (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).

    Article Snippet: A surface object workflow was used in Imaris to define the DAPI+ nuclear ROI.

    Techniques: Derivative Assay, Staining, Mutagenesis